Different Types of Cell Banks for Stroke Research

Stem Cell Bank

Ace Neuroscience has an extensive cell bank covering the types of stem cells that can be used to develop stem cell therapy and other studies, including but not limited to adult tissue-derived stem cells, adipose tissue-derived MSCs, breastmilk-derived stem cells, and menstrual blood stem cells. Based on our stem cell bank, our expert scientists will select the most suitable type of stem cell for you based on your experiment.

Brain Cell Bank

To some extent, the pathological state of the brain after a stroke can be reflected by in vitro studies of different brain cells. Ace Neuroscience provides neurons, epithelial cells, and neuroglia, such as astrocytes, oligodendrocytes, and microglia.

• Glial cells mediate multiple physiological and pathological responses to the stroke process, including oxidative stress, inflammatory responses, neuroprotection, and neurotrophic effects.
• Epithelial cells can measure vascular changes after stroke.
• Neurons play a vital role in the brain. The target of many stroke therapies is by protecting or restoring neuronal function.

Different Sources of Cell Banks for Stroke Research

Cell banks from different sources play different roles at different stages of stroke research. Therefore, it is important for stroke research to establish a cell bank covering different mammalian, insect, microbial, and other cells.

Mammalian, Insect & Other Cell Banks

Ace Neuroscience has a variety of mammalian, insect, avian, and other types of cells and has constructed cell banks accordingly. We rigorously test the viability of the cells after banking, sterility, and mycoplasma. For cell expansion for testing, we provide in vivo adventitious agent tests, MAP and HAP tests, TEM tests, bovine and porcine adventitious agent tests, and other relevant tests.

• Our microbial contamination assays include bacteriostatic and fungistasis, sterility, mycoplasma, spiroplasma, and mycobacterium.
• We test cells for virological contamination by transmission electron microscopy, reverse transcriptase PCR assay, retroviral infectivity assays, in vitro and in vivo adventitious virus tests, PCR and in vitro assays for specific viruses, and other assays.
• We perform identification and genetic stability analysis of cell lines by isoenzyme analysis and karyology, DNA barcoding or other DNA fingerprinting methods, copy number determination, restriction endonuclease analysis, DNA / RNA sequencing, and other methods.

Microbial Cell Banks

Ace Neuroscience has extensive experience in manufacturing microbial cell banks. We always hold ourselves to the highest standards and strictly control every step of the manufacturing and shipping process to ensure the quality and biosafety of our cells.

• For the production and release of cell bank, we perform strict bacteriophage, viability, purity, and identification tests to ensure that the cells are free of contamination.
• We further perform tests related to the retention of selectable markers, retention of recombinant structures, sequencing, restriction endonuclease analysis, copy number determination, and other relevant tests on microbial cells to provide comprehensive information on the cells and ensure the safety of the cell bank.

Cell Bank Storage, Retrieval & Distribution

Storage Ace Neuroscience strictly manages and classifies cells. We store cells at -70°C or in liquid nitrogen.

Retrieval Ace Neuroscience assigns a unique identification number to each vial of cells for fast and accurate retrieval.

Distribution Ace Neuroscience transfers the removed cells to a special transport container, which is equipped with a recording device to monitor the cell transport environment.

Introduction of Animal Models in Psychiatry

Neuropsychiatric disorders such as schizophrenia, major depressive disorder, bipolar disorder, and autism are so prevalent, begin early in life, and have a major impact on the worldwide disease burden that new therapeutic mechanisms have all but stalled. It is difficult to imagine major advances in pathophysiology or therapeutics without good animal models.

Animal Modeling Services for Psychiatric Diseases

Ace Neuroscience has established a variety of animal models of mental illness through methods such as selective breeding, genetic engineering, brain injury, and environmental manipulation. These models simulate as much as possible the neurological or behavioral pathology of human diseases. These models can help you understand disease pathophysiology and accelerate the development of therapeutics based on new molecular targets.

• Depression Animal Models

We have developed a variety of animal models based on the etiology of depression, acute or chronic stress exposure, gene environment interaction, exogenous glucocorticoid administration and gene manipulation. These animal models of depression can be used to study the molecular and cellular pathways that may be crucial to the pathogenesis of depression.

• Schizophrenia Animal Models

We have developed more than 20 different animal models of schizophrenia based on developmental, drug-induced, lesion and genetic manipulation. These models are very valuable preclinical tools that can be used to help you study the neurobiological basis of schizophrenia.

• Anxiety Animal Models

We have established a variety of animal models of anxiety disorders through psychosocial, physical and chronic unpredictable stress. These models are helpful to study the pharmacological mechanism and potential clinical effects of drugs.

• Addiction Animal Models

Our addiction models are excellent tools for identifying pathological mechanisms, target recognition, and drug development, and can provide critical insights into associative learning, functional neuroanatomy, and cellular mechanisms of drug addiction disorders.

• Bipolar Disorder Animal Models

We offer a selection of pharmacological, environmental and genetic animal models for the study of bipolar disorder, the vast majority of which recapitulate specific aspects associated with mania.

• Autism Animal Models

We have established a variety of animal models of autism by mutating homologous genes in experimental animals, and these models can be used to analyze the role of many genetic abnormalities in the etiology and symptomology of autism spectrum disorder.

• Obsessive–compulsive Disorder Animal Models

We have developed multiple animal models of OCD through genetic, pharmacologically induced, behavior-based manipulation, and neurodevelopmental approaches that can be used to develop new therapeutic strategies for OCD and other related disorders.

• Eating Disorders Animal Models

We can provide some environmental and genetic animal models for studying eating disorders, including animal models of anorexia nervosa and animal models of bulimia nervosa/binge eating. These models accurately reproduce the alterations in feeding and neuroendocrine function that are unique to each eating disorder.

Different Types of Superbugs

Some of the antibiotic-resistant pathogens that we currently face are classified into different threat levels. Some superbugs are considered to be an urgent threat to us, including Carbapenem-resistant Acinetobacter, Carbapenem-resistant Enterobacteriaceae (CRE), Clostridioides difficile, etc. Pathogens of serious threat level include Multidrug-resistant Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus, etc. Pathogens of concern level include some drug-resistant Streptococcus.

Some superbugs that are common in clinical practice include Carbapenem-resistant Enterobacteriaceae, Methicillin-resistant Staphylococcus aureus (MRSA), and Vancomycin-resistant Enterococci (VRE), which can cause a variety of serious infections.

Molecular biology analysis in psychiatry is an approach that relies on biological techniques at the molecular level to study psychiatric disorders. It includes the study of expression profiles, DNA, RNA, proteins, and metabolites at the molecular level.

Our molecular biology analysis can also be applied to study the development of novel drugs. For example, by modulating one or more genes, proteins, and metabolic pathways to develop a novel drug and analyze its effects on the nervous system.

Molecular Biology Services

• Gene Expression Analysis in Psychiatry

We provide gene expression analysis services related to psychiatric disorders to support research in gene expression, RNA (transcriptome) analysis, biomarker identification and validation, and biologics safety testing. Our gene expression analyses help you understand the mechanisms of psychiatric disorders and promote the development of precision treatment strategies for psychiatric disorders.

• Protein Analysis in Psychiatry

We provide protein analysis services for the study of various psychiatric disorders. By studying how drugs bind to specific proteins and the mechanisms that influence their function, we can provide guidance and rationale for the research and design of new drugs. In addition, we can help you understand the mechanisms of signal transduction, transmembrane transport, cellular metabolism, and protein interaction-related biological processes related to psychiatric pathology, thus helping you identify biomarkers and unravel the pathogenesis of psychiatric disorders.

• Autoradiography Services in Psychiatry

We provide specialized services for radioligand binding assays, receptor occupancy studies, and radioautography for the study of psychiatric disorders. We provide a comprehensive assessment of the pathophysiology of psychiatric disorders and data on the mechanism of action of therapeutic compounds through radiographic autoradiography combined with behavioral readings.

• Oxidative Stress Assays in Psychiatry

We offer a variety of oxidative stress assays for tracking different parameters of oxidative stress as well as for detecting the status and activity of key oxidative stress biomarkers. We are committed to helping researchers better monitor the mechanisms and responses to oxidative stress associated with psychiatric disorders.

• Neurotransmitter Analysis in Psychiatry

Neurotransmitters are chemicals in the brain responsible for neural signaling and play an important role in the pathogenesis of psychiatric disorders. We were able to analyze a wide range of neurotransmitters from a large number of biological matrices through an electrochemical platform and subsequent neurochemical bioanalytical tools.

Different Types of Cell Banks for Stroke Research

Stem Cell Bank

Ace Neuroscience has an extensive cell bank covering the types of stem cells that can be used to develop stem cell therapy and other studies, including but not limited to adult tissue-derived stem cells, adipose tissue-derived MSCs, breastmilk-derived stem cells, and menstrual blood stem cells. Based on our stem cell bank, our expert scientists will select the most suitable type of stem cell for you based on your experiment.

Brain Cell Bank

To some extent, the pathological state of the brain after a stroke can be reflected by in vitro studies of different brain cells. Ace Neuroscience provides neurons, epithelial cells, and neuroglia, such as astrocytes, oligodendrocytes, and microglia.

• Glial cells mediate multiple physiological and pathological responses to the stroke process, including oxidative stress, inflammatory responses, neuroprotection, and neurotrophic effects.
• Epithelial cells can measure vascular changes after stroke.
• Neurons play a vital role in the brain. The target of many stroke therapies is by protecting or restoring neuronal function.

Different Sources of Cell Banks for Stroke Research

Cell banks from different sources play different roles at different stages of stroke research. Therefore, it is important for stroke research to establish a cell bank covering different mammalian, insect, microbial, and other cells.

Mammalian, Insect & Other Cell Banks

Ace Neuroscience has a variety of mammalian, insect, avian, and other types of cells and has constructed cell banks accordingly. We rigorously test the viability of the cells after banking, sterility, and mycoplasma. For cell expansion for testing, we provide in vivo adventitious agent tests, MAP and HAP tests, TEM tests, bovine and porcine adventitious agent tests, and other relevant tests.

• Our microbial contamination assays include bacteriostatic and fungistasis, sterility, mycoplasma, spiroplasma, and mycobacterium.
• We test cells for virological contamination by transmission electron microscopy, reverse transcriptase PCR assay, retroviral infectivity assays, in vitro and in vivo adventitious virus tests, PCR and in vitro assays for specific viruses, and other assays.
• We perform identification and genetic stability analysis of cell lines by isoenzyme analysis and karyology, DNA barcoding or other DNA fingerprinting methods, copy number determination, restriction endonuclease analysis, DNA / RNA sequencing, and other methods.

Microbial Cell Banks

Ace Neuroscience has extensive experience in manufacturing microbial cell banks. We always hold ourselves to the highest standards and strictly control every step of the manufacturing and shipping process to ensure the quality and biosafety of our cells.

• For the production and release of cell bank, we perform strict bacteriophage, viability, purity, and identification tests to ensure that the cells are free of contamination.
• We further perform tests related to the retention of selectable markers, retention of recombinant structures, sequencing, restriction endonuclease analysis, copy number determination, and other relevant tests on microbial cells to provide comprehensive information on the cells and ensure the safety of the cell bank.

Cell Bank Storage, Retrieval & Distribution

Storage Ace Neuroscience strictly manages and classifies cells. We store cells at -70°C or in liquid nitrogen.

Retrieval Ace Neuroscience assigns a unique identification number to each vial of cells for fast and accurate retrieval.

Distribution Ace Neuroscience transfers the removed cells to a special transport container, which is equipped with a recording device to monitor the cell transport environment.

Bacteria are becoming resistant to antibiotics at an alarming rate, posing a major threat to our health care. Rapidly identifying the bacteria that cause specific infections is critical to preventing the development of antibiotic resistance. However, many researchers may face some challenging processes in superbug identification techniques.

Rapid Detection of Superbugs Using Phages

Phages offer a unique opportunity for superbug detection. Using the ability of phages to specifically target specific strains of bacteria, it may can be as superbug cell recognition elements. Phage particles are able to specifically recognize surface receptors on host bacterial cells and bind, leading to early and rapid detection and identification of drug-resistant pathogens.

Given the unique cellular sensitivity characteristics of phages, they can be used to create an effective identification method for drug-resistant bacteria. We offer services for the development of reporter phage-based superbug detection systems that take advantage of their rapid and sensitive properties making them ideal tools for detecting drug-resistant bacteria.

Rapid Detection of Superbugs by Specific Enzyme Identification

There has been found that one of superbugs' main defense mechanisms for developing antibiotic resistance is the production of resistant enzymes such as β-lactamases (Blas). For drug-resistant bacteria, we need not only to investigate novel antibiotics that target Blas, but also to develop new enzyme assays to identify superbugs.

As these types of enzymes continue to emerge, methods for selective and specific detection of enzymes have been developed and have become important diagnostic tools for the detection and monitoring of drug-resistant bacteria. We provide services to develop new enzyme assays to identify superbugs, which mainly target drug-resistant enzymes, such as β-lactamases.

Rapid Detection of Superbugs Using DNA Nanosensor

DNA-based nanosensors generally use DNA as a recognition probe. For known superbugs, specific DNA sequences can be developed as recognition elements to specifically detect pathogens in the sample. We can create DNA/RNA signatures to precisely diagnose and identify specific bacteria. We are committed to the goal of rapid detection of superbugs and offer development services for DNA nanosensors that can reliably detect target superbugs and unknown pathogens.

Rapid Detection of Superbugs Using CRISPR-Cas System

Diagnostic technologies based on the CRISPR-Cas system have been rapidly developed in recent years. These systems also can be designed to detect superbugs quickly and reliably. CRISPR-Cas systems can be used to detect nucleic acids and biomarkers in bacteria or viruses. For extracted and amplified pathogenic nucleic acids, the corresponding CRISPR-Cas system can recognize and cleave them. Then, quantitative detection of pathogens is achieved by fluorescence or other methods.