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Introduction to Genetic CircuitSynthetic biology is a field of science that deals with engineering and redesigning biological systems. The design of genetic circuits is inspired by electronics and computer science. Genetic circuits can perform logical functions mimicking those observed in electronic circuits and exhibit control over the flow of information in biological systems. Genetic circuit design should follow the principles of modularity, orthogonality, predictability, and reliability.In recent years, progress in genetic engineering makes it possible to construct novel genetic circuits with different functions and activation mechanisms. Moreover, the development of new tools, including high-throughput DNA assembly and characterization, computer-aided design, and design automation software, enables rapid and automated design and assembly of genetic circuits. These have the potential to accelerate the design of larger gene circuits, thereby increasing our understanding of circuits and host behavior. 

Introduction to Protein DesignProtein design aims to create proteins with enhanced or novel activity, behavior, or other functional properties. As a powerful tool, it may have a profound impact on synthetic biology and may have more rapid and innovative applications. In the near future, protein design, especially computational design, will greatly expand the functional capabilities of synthetic cells. De Novo Protein DesignIn de novo protein design, the ultimate objective is to identify the appropriate amino acid sequence that best fits the designed topology of protein with the desired functions. We are dedicated to establishing the most exquisite service platform for our clients. Our one-stop services can provide comprehensive technical support to advance our customers' projects.Rational Protein DesignRational protein design plays a critical role in understanding protein structure and physical chemistry. It has been widely used in numerous fields, including synthetic biology. Based on the understanding of the target protein's structure and the correlated properties, our professional scientific team can provide our customers with a complete development cycle of rational design.Semirational Protein DesignAs a combination of rational design and directed evolution, semirational protein design has been developed to address the limitations of both methods. Information on protein sequence, structure and function is used together with predictive algorithms to identify target amino acid residues. We provide multiple approaches to bring diverse synthetic applications and creative ideas to fruition.

The ability to overexpress genes in microorganisms has revolutionized the field of synthetic biology. Gene overexpression involves the production of large quantities of target proteins by increasing the transcription and translation of the gene of interest. This technique has been applied in the engineering of microorganisms to produce a wide range of products, including biofuels, chemicals, and biopharmaceuticals. There are several strategies for gene overexpression in strain engineering. One common method is gene synthesis, which involves the design and synthesis of genes optimized for expression in specific host organisms. Gene experts modify the codon usage, remove destabilizing sequences, and add regulatory elements to enhance expression. Therefore, the target expression level of the gene is achieved through the modification and design of the gene. Another approach is cloning. Experts utilize state-of-the-art techniques, such as restriction enzyme digestion and DNA ligation, polymerase chain reaction (PCR) amplification, plasmid-mediated transformation, and gene transfection, to clone synthesized genes into different expression vectors, enabling high levels of gene expression.   In some cases, to enhance gene expression, regulatory elements that may reduce gene expression levels can be identified and removed. This type of gene optimization can be achieved by modifying the promoter, ribosome binding site, and other regulatory elements to obtain optimal gene expression levels. Overall, gene overexpression is a powerful tool in strain engineering to enhance the production of specific compounds or metabolic activities. It involves increasing the expression level of specific genes through techniques such as gene synthesis, cloning, gene optimization, and protein expression. From a synthetic biology perspective, researchers can explore the role of gene overexpression in strain engineering and how they can optimize gene overexpression for diverse applications.

Cell-free protein expression or cell-free protein synthesis (CFPS) refers to the protein expression approach that produces target proteins without using living cells. All the cellular machinery, such as ribosomes, enzymes, tRNAs, amino acids, and cofactors, are contained in CFPS for direct protein synthesis. A supplied nucleic acid template can be transcribed and translated in CFPS, and this process is not constrained by a cell wall or homeostasis conditions required to maintain cell viability.CFPS is well-suited for many applications. It has recently evolved into key platforms for synthetic biology applications. The cell-based systems have limited applications, while CFPS can circumvent many limitations and eliminate complexities. It provides exciting opportunities for rational design and manipulation of biological systems.

Introduction of Strain HybridizationOne of the key components of synthetic biology is the creation of novel strains with unique properties that can be used for various applications such as biotechnology, medicine, and agriculture. Strain hybridization is an essential technique in synthetic biology that involves the combination of genetic material from different strains to create a new hybrid strain with desired traits.  

The Synthetic Biology Platform is designed to program comprehensive biosynthesis development in one place. The goal was to develop a platform that could program comprehensive biosynthesis development in one place, and since then, it has become a reality. The Synthetic Biology Platform is a disruptive technology platform focusing on synthetic biology, which can contribute to the advancement of diverse areas, including but not limited to industrial biotechnology, pharmaceutical, agriculture, and healthcare research. The Synthetic Biology Platform integrates engineering principles and a broad range of methodologies from various disciplines, such as biology, bioinformatics, chemistry, physics, mathematics, and computer science. It’s designed to perform strain-based chassis engineering as well as biosynthesis development for various molecules, thus gaining insights into disease mechanisms, identifying novel drug targets, and accelerating drug discovery and development. Combining the very robust technologies of CRISPR, microfluidic, nanotechnology, omics, and bioinformatics, the Synthetic Biology Platform reaches beyond traditional technology applications, providing cutting-edge synthetic and analytical tools, as well as elucidating the biology and chemistry functionalities. Instead of following a narrow, vertical structure, it embraces a platform-based model, and its synthetic biology toolboxes can help researchers accelerate the design and construction of new biological entities or the re-design of natural biological processes and pathways through the iterative Design-Build-Test-Learn (DBTL) cycles when utilizing the Synthetic Biology Platform. The Synthetic Biology Platform offers reliable biosynthesis development for a large selection of chemicals, natural compounds, industrial enzymes, etc., which could seamlessly integrate into the subsequent workflows, thus greatly accelerating workflow efficiency. In addition, the Synthetic Biology Platform is able to achieve appropriate chassis engineering for gene editing, gene mutation, and many other applications. More importantly, it is capable of designing, building, testing, and learning together to streamline and standardize the whole project process, including host selection, modeling, genetic engineering, sequencing, and data analysis. Not to mention, the Synthetic Biology Platform consists of modules designed to provide scientists with an intuitive, easy-to-use, and comprehensive set of tools that come seamlessly together to form an enterprise-ready scientific informatics platform. With powerful capabilities and flexibility, it can support and facilitate the applications of synthetic biology in a wide range of areas. Meanwhile, it also can be customized to tailor solutions according to researchers’ unique and innovative demands. In other words, everything they need – in one place. About the Synthetic Biology PlatformSynthetic Biology Platform is a state-of-the-art solution platform for scientific research and discovery. Its flexibility can meet all demands of the synthetic biology industry and those of various business units in R&D organizations today, thus rapidly adapting and scaling to meet whatever requirements researchers may raise.

The Synthetic Biology Platform is a comprehensive toolbox and fully integrated platform to empower synthetic biology research and development. The Synthetic Biology Platform is a comprehensive toolbox and fully integrated platform to empower synthetic biology research and development. Synthetic biology is an emerging interdisciplinary field that combines principles of engineering and biology to create artificial lives or re-design organisms for useful purposes. In fact, designing a platform that could carry out full-scale biosynthesis development from chemicals to enzymes in one place can save much cost and time for synthetic biology researchers. Given the need, a cutting-edge Synthetic Biology Platform is developed to offer technical support for the development of synthetic biology applications. The Synthetic Biology Platform integrated with the CRISPR technology can regulate gene expression needed for synthetic biology more efficiently and precisely. Complex genetic circuits based on CRISPR allow for more accurate regulation of gene expression in terms of expression levels and timing than traditional methods. Besides, combined with microfluidic technologies, the Platform allows for precise environmental control while capturing high-quality data. Ever since the introduction of the first synthetic genetic circuit, the development of synthetic biology and microfluidics have been intertwined. Hence, in utilizing the Synthetic Biology Platform, researchers can not only achieve low-cost and high-yield biosynthesis of specific products by incorporating a precision fermentation process but also take advantage of the robust integrated tools, such as omics, bioinformatics, and nanotechnology, that elevate the Synthetic Biology Platform beyond conventional technological applications. With powerful capabilities and flexibility, on the one hand, the Synthetic Biology Platform can select an appropriate framework for research, including minimal genomes, bacterial chassis, cell-free synthetic biology chassis, and many others, for the next generation of synthetic biology chassis to display biological complexity and predict behaviors. On the other hand, the platform can improve the yield of chemicals, amino acids, peptides, proteins, and enzymes using genome editing, microbial fermentation, and many other synthetic biology strategies. Synthetic Biology Platform is able to offer a wide range of enabling technologies and a comprehensive synthetic biology toolbox to help researchers accelerate the design and construction of new biological entities or the re-design of natural biological processes through the iterative Design-Build-Test-Learn (DBTL) cycles of synthetic biology. This system is dedicated to assisting synthetic biology researchers around the world with custom synthetic biology services and effective strategies. About the Synthetic Biology PlatformThe Synthetic Biology Platform is a state-of-the-art solution platform for scientific research and discovery. Its flexibility can meet all demands of the synthetic biology industry and those of various business units in R&D organizations today, thus rapidly adapting and scaling up to meet whatever requirements researchers may raise.