Amerigo Scientific offers four series of ion exchange (IEX) chromatography resins of different matrix type and pore size to satisfy a variety of applications. These products are complementary with each other and are solutions of all process steps (capture, intermediate purification, and polishing) in chromatography for purifications of a variety of substances from small peptides to large proteins and viral particles.Uni Series IEX ResinsUniCM, UniSP, UniQ, UniDEAE, and UniMSP are designed for the IEX purification of small proteins, peptides, oligo nucleotides, and small molecule such as antibiotics. Their supports are highly crosslinked polymethacrylate beads of uniformed particle size. Uni IEX resins have good hydrophilicity and low non-specific adsorbability by surface modification. Their smaller pore size makes them more suitable for IEX purification of small biomolecules. All of these resins meet the reproducibility, extensibility, and reliability requirements of today's industrial biological processes.
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High-performance liquid chromatography (HPLC) columns are used for the analysis or preparation of separated compounds from complex mixtures. The pressurized liquid solvent containing the sample mixture passes through a column filled with a solid adsorbent material. Since each component in the sample interacts with the adsorbent material slightly differently, the components with different flow rates are separated as they flow out of the column. According to separation mechanism, common HPLC column types are classified as normal/reversed phase, mixed-mode, ion exchange, ligand exchange, and affinity column formats.HPLC CartridgeThe physical properties of the target component are the decisive factor in the selection of a column. The molecular characteristics that influence HPLC column selection include molecular size, hydrophobicity/hydrophilicity, intermolecular force, and intramolecular force. In addition, the inner diameter and volume of the column determine how much sample that can be loaded on the column and the sensitivity of the separation.
Laboratory equipment is a general term for the various instruments, containers and other tools needed for operation, synthesis, and analysis in various laboratories. The equipment helps researchers to observe, test, experiment or analyze data to confirm the authenticity or rationality of scientific hypotheses. According to the scope of application, laboratory equipment can be divided into general equipment and specialized equipment.General laboratory equipment includes freezers, incubators, shakers, analytical balances, pipettes, pH meters, microscopes, consumables, etc. More specialized laboratory equipment includes autoclave, cell culture equipment, PCR machine and mass spectrometer, flow cytometer, imaging system, analysis software, etc. With the rapid development of technology, more and more innovative automatic instruments have come out to solve experimental problems with their unique design, complete functions, and simple operation.
Strong base anion (SBA) exchange resin is one type of ion exchange resins containing quaternary ammonium groups on the microsphere surfaces. The SBA resin shows an excellent physical and chemical stability, which is capable to exchange the different anion under a wide range of pH value. SBA resins are ideal for removing certain anionic contaminants and can be regenerated with salt.SBA resins are usually divided into two classes according to their functional groups. Type I SBA resins are aminated with trimethylamine resulting a trimethyl ammonium group. Type II SBA resins are aminated with dimethyl-ethanolamine resulting a dimethylethanol ammonium group. Type I SBA resins provide lower total organic carbon (TOC) effluents than type II SBA resins because of the stronger chemical stability of type I resins. In addition, type I SBA resins are ideal when the goal is to achieve low silicon leakage because type I SBA resins can be regenerated at the high temperatures required for silicon to eluate from the resins. However, type II SBA resins have better regeneration efficiencies and higher operating capacities than type I SBA resins and are more suitable for applications needing good rinsing performance and excellent regeneration efficiency.
The importance of nucleic acids in controlling life processes is well known. Under the suitable conditions, DNA molecules are responsible for replication of themselves and acts as templates for RNA synthesis in transcription. In translation, RNA molecules control protein synthesis and thus various metabolic activities in living cells. All these processes are monitored by sequences of bases in polynucleotide chains. Nucleosides consist of a sugar group and a heterocyclic nucleobase that bound to the sugar group by a P-N-glycosidic linkage. The sugar group in the ribonucleosides is a D-ribose, and that in the deoxyribonucleosides is a 2‘-deoxy-Dribose. The nucleobases are amino- and hydroxyl-substituted purine and pyrimidine derivatives, including adenine (A), guanine (G), cytosine (C), uracil (U), thymine (T). The corresponding ribonucleosides are designated adenosine (rA), guanosine (rG), cytidine (rC), uridine (rU). Deoxyribonucleosides are prefixed by deoxy- and their abbreviations are dA, dG, dC, dT. In addition to being building blocks of DNA and RNA, nucleosides play key roles in neurotransmission, signaling transduction, and regulation of cardiovascular activity. Because of their ability to interfere with the synthesis of nucleic acids and cellular repair systems, nucleoside derivatives serve as anti-tumor agents and as antimicrobials. In addition, nucleoside molecules have been developed for use in agricultural chemistry as herbicides or insecticides.The nucleotides are phosphorylated nucleosides that contain an additional phosphoester group at the 3'- or 5'-hydroxyl function of the sugar group. Nucleotides and their derivatives are involved in multiple important biological processes. They play diverse roles in energy metabolism, enzymatic regulation, and signal transduction and serve as structural components of coenzymes. Nucleoside triphosphates and nucleoside diphosphate, especially adenosine triphosphate (ATP) and adenosine diphosphate (ADP), facilitate energy transfer in biological systems. ATP and ADP also play key roles in the phosphorylation of various enzymes. Adenine nucleotides are components of various coenzymes such as nicotinamide adenine dinucleotide (NAD), flavin adenine dinucleotide (FAD), and coenzyme A (CoA). Nucleotides also act as mediators in metabolic regulation, such as the cyclic nucleotides cyclic adenosine monophosphate (cAMP) and guanosine monophosphate (cGMP) as messengers of hormonal regulation. Adenosine Monophosphate (AMP), ADP, cytidine triphosphate (CTP), and guanosine triphosphate (GTP) are allosteric regulators of enzymes. GTP and Guanosine diphosphate (GDP) are active in signal transduction cascades.
Bioink is a solution of a biomaterial or a mixture of several biomaterials in the hydrogel form, usually encapsulating living cells, and/or bioactive molecules. For bioprinting, bioinks are essential components, and are cross-linked or stabilized during or immediately after the bioprinting to create the final shapes of the intended tissue constructs.The selection of appropriate bioink depends on the specific application and the type of cells as well as the bioprinter to be used. An ideal bioink should possess the desired physicochemical properties, such as cross-linking capability, mechanical, rheological, chemical, and biological characteristics.
Laboratory equipment is a general term for the various instruments, containers and other tools needed for operation, synthesis, and analysis in various laboratories. The equipment helps researchers to observe, test, experiment or analyze data to confirm the authenticity or rationality of scientific hypotheses. According to the scope of application, laboratory equipment can be divided into general equipment and specialized equipment.General laboratory equipment includes freezers, incubators, shakers, analytical balances, pipettes, pH meters, microscopes, consumables, etc. More specialized laboratory equipment includes autoclave, cell culture equipment, PCR machine and mass spectrometer, flow cytometer, imaging system, analysis software, etc. With the rapid development of technology, more and more innovative automatic instruments have come out to solve experimental problems with their unique design, complete functions, and simple operation.
